| Issue | #26 |
| Published | July 1963 |
| Cover Price | 0.12 USD |
| Pages | 36 |
| Editing | Victor Gorelick |
| Characters | The Fly; The Spider |
| Genre | superhero |
| Pencils | John Rosenberger |
| Inks | John Rosenberger |
| Characters | The Fly; The Spider; "Big Joe" Saxton (a mobster) |
| Synopsis | After he's captured by the Fly, "Big Joe" Saxton springs the Fly's foe the Spider free from jail in order to defeat the hero once and for all. |
| Genre | superhero |
| Script | Robert Bernstein |
| Pencils | John Rosenberger |
| Inks | John Rosenberger |
| Notes | Story continues in the next sequence. |
| Characters | The Fly; Fly Girl; The Spider; "Big Joe" Saxton |
| Synopsis | Fly Girl inadvertently aids the Fly in escaping from the Spider's trap. But the hero decides to take down the wily criminal mastermind alone. |
| Genre | superhero |
| Script | Robert Bernstein |
| Pencils | John Rosenberger |
| Inks | John Rosenberger |
| Characters | Black Hood |
| Genre | superhero |
| Script | Robert Bernstein |
| Pencils | John Rosenberger |
| Inks | John Rosenberger |
| Characters | The Fly |
| Synopsis | After being shown a top-secret government defense against an atomic bomb, the Fly seemingly goes insane and destroys the project. |
| Genre | superhero |
| Script | Robert Bernstein |
| Pencils | John Rosenberger |
| Inks | John Rosenberger |