| Issue | #21 |
| Published | September 1962 |
| Cover Price | 0.12 USD |
| Pages | 36 |
| Editing | Victor Gorelick |
| Characters | The Fly; Fly Girl |
| Genre | superhero |
| Pencils | John Rosenberger |
| Inks | John Rosenberger |
| Characters | Fly; Fly Girl |
| Synopsis | The Fly and Fly Girl recover a United States rocket from an alien planet where gigantic germs are the primary life form. |
| Genre | superhero |
| Script | Robert Bernstein |
| Pencils | John Rosenberger |
| Inks | John Rosenberger |
| Characters | Fly; Fly Girl; Anti-Fly League [Spider; Bra-Kr; Metal Monster; Roxr; Lxo III; Dovi; Karl Rance; Linda] |
| Synopsis | Previous foes of the Fly and Fly Girl form the Anti-Fly League with one goal in mind: the destruction of the two heroes. |
| Genre | superhero |
| Script | Robert Bernstein |
| Pencils | John Rosenberger |
| Inks | John Rosenberger |
| Characters | Fly; Fly Girl |
| Genre | superhero |
| Letters | typeset |
| Characters | Cat Girl; Jill Ross; Kree-Nal; Ralph Hardy |
| Genre | superhero |
| Characters | Fly Girl |
| Synopsis | Fly Girl aids the FBI by doing public relations work for a textile firm in order to find a Soviet spy ring. |
| Genre | superhero |
| Script | Robert Bernstein |
| Pencils | John Rosenberger |
| Inks | John Rosenberger |
| Notes | It is revealed that Fly Girl is color blind in this issue. |