| Issue | #100 |
| Published | January 2007 |
| Cover Price | 3.50 USD |
| Pages | 36 |
| Editing | Diana Schutz (editor); Dave Marshall (assistant editor) |
| Notes | This special issue has eight extra pages and a card stock wraparound cover, for a higher price. |
| Characters | Miyamoto Usagi; Tomoe Ame; Murakami Gennosuke; Jotaro; Inspector Ishida; Katsuichi-Sensei |
| Synopsis | The cast ascends a bluff, gazing toward the future. |
| Genre | funny animals; adventure; historical |
| Pencils | Stan Sakai (signed) |
| Inks | Stan Sakai (signed) |
| Colors | Tom Luth (signed) |
| Letters | typeset |
| Notes | This is a wraparound cover, encompassing the front and back covers. |
| Synopsis | Stan Sakai outlines the origins and publishing history of his character, Usagi Yojimbo. |
| Script | Stan Sakai |
| Letters | typeset |
| Notes | Inside front cover. |
| Characters | Mike Richardson; Stan Sakai; Diana Schutz; Concrete; The Mask; Rick Geary |
| Synopsis | Dark Horse Publisher Mike Richardson outlines what to expect in this special anniversary issue. |
| Genre | Humor |
| Script | Mike Richardson |
| Pencils | Rick Geary |
| Inks | Rick Geary |
| Letters | Rick Geary |
| Characters | Miyamoto Usagi; Marv |
| Synopsis | Marv from the Sin City comic rescues Usagi from a barbecue grill. |
| Genre | Humor |
| Script | Frank Miller |
| Pencils | Frank Miller |
| Inks | Frank Miller |
| Letters | Frank Miller |
| Characters | Miyamoto Usagi; Concrete; various Dark Horse staffers and freelancers. |
| Synopsis | Usagi makes his escape from the Celebrity Roast. |
| Genre | Humor |
| Script | Diana Schutz |
| Pencils | Matt Wagner |
| Inks | Matt Wagner |
| Letters | Tom Orzechowski |
| Characters | Stan Sakai; Sergio Aragones |
| Synopsis | Stan gives his opening remarks to the banquet guests. |
| Genre | Humor |
| Script | Stan Sakai |
| Pencils | Stan Sakai |
| Inks | Stan Sakai |
| Letters | Stan Sakai |
| Characters | Sergio Aragones; Stan Sakai; Mark Evanier; Miyamoto Usagi; Mike Richardson; Diana Schutz; other Dark Horse staffers and contributors |
| Synopsis | Sergio Aragones relates a humorous anecdote about a trip he and Stan took to Hawaii. |
| Genre | Humor |
| Script | Sergio Aragones |
| Pencils | Sergio Aragones |
| Inks | Sergio Aragones |
| Letters | Stan Sakai |
| Characters | James S. Rich; Ben Abernathy; Stan Sakai; Miyamoto Usagi; Andi Watson |
| Synopsis | James Rich relates a humorous anecdote about Stan Sakai. |
| Genre | Humor |
| Script | James S. Rich |
| Pencils | Andi Watson |
| Inks | Andi Watson |
| Letters | Andi Watson |
| Characters | Stan Sakai |
| Synopsis | Transition page between anecdotes. |
| Genre | Humor |
| Script | Stan Sakai |
| Pencils | Stan Sakai |
| Inks | Stan Sakai |
| Letters | Stan Sakai |
| Characters | Jeff Smith; Stan Sakai; unnamed comic convention guests |
| Synopsis | Jeff Smith relates a convention anecdote about Stan Sakai. |
| Genre | Humor |
| Script | Jeff Smith |
| Pencils | Jeff Smith |
| Inks | Jeff Smith |
| Letters | Jeff Smith |
| Characters | Mark Evanier; Scott Shaw; Mike Richardson; Stan Sakai; Hannah Sakai; Ricky Rabbit; Harry Hare; The Old Gray Hare; Rapid-Fire Rabbit; Bongwater Bunny; Floppy-Ears Fanbunny; Captain Carrot (in silhouette); Linda Lapin; Hello Rabbitty |
| Synopsis | Mark Evanier and Scott Shaw bring out a host of other comic book rabbits to speak about the anniversary. |
| Genre | Humor |
| Script | Mark Evanier |
| Pencils | Scott Shaw! |
| Inks | Scott Shaw! |
| Letters | Scott Shaw! |
| Characters | Stan Sakai; Guy Davis |
| Synopsis | Stan relates his first meeting with artist Guy Davis. |
| Genre | Humor |
| Script | Stan Sakai |
| Pencils | Stan Sakai |
| Inks | Stan Sakai |
| Letters | Stan Sakai |
| Characters | Guy Davis; Stan Sakai; Miyamoto Usagi; various Komori Ninja; Lone Goat & Kid |
| Synopsis | While Guy Davis talks, Stan Sakai dozes off and dreams of a mini-Usagi Yojimbo adventure with a twist ending. |
| Genre | Humor |
| Script | Guy Davis |
| Pencils | Guy Davis |
| Inks | Guy Davis |
| Characters | Stan Sakai; Miyamoto Usagi; Tomoe Ame; Jotaro; Lord Noriyuki; Murakami Gennosuke; Inspector Ishida; Katsuichi-Sensei; Zato Ino; Kenichi; Mariko; Kitsune; Lone Goat & Kid; The Woodcutters; Chizu; Inazumi; Mike Richardson; Sergio Aragones; other Dark Horse staffers and contributors |
| Synopsis | Stan decides that things have gone far enough and sends his characters after the guests. |
| Genre | Humor |
| Script | Stan Sakai |
| Pencils | Stan Sakai |
| Inks | Stan Sakai |
| Letters | Stan Sakai |